Protein Purification Reagents and Kits
ABclonal Technology DDDDK-Tag Rabbit mAb
The FLAG-tag (DYKDDDDK) is a short polypeptide added to proteins via recombinant DNA technology. It is widely used for protein purification by affinity chromatography and for studying proteins in living cells. The tag helps isolate recombinant proteins from host proteins and enables purification of multi-subunit complexes without disrupting them. It is also used to obtain proteins suitable for x-ray crystallography. The FLAG-tag can be detected with specific antibodies, making it useful in assays like immunofluorescence, SDS-PAGE, and Western blotting, especially when no antibody exists for the target protein.
ABclonal Technology 450 Rabbit anti-Human CD45RA
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitosis and oncogenic transformation This PTP contains an extracellular domain a single transmembrane segment and two tandem intracytoplasmic catalytic domains and thus is classified as a receptor type PTP This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling It functions through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling This PTP also suppresses JAK kinases and thus functions as a regulator of cytokine receptor signaling Alternatively spliced transcripts variants of this gene which encode distinct isoforms have been reported
Invent Biotechnologies Inc Minute™ Nuclear Envelope Protein Extraction Kit - Spin Column-based (50 Preps)
The nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify because of its connection to nuclear and cytoplasmic components. Traditional methods of nuclear envelope isolation and purification require a large amount of starting material and a long, tedious procedure. Minute™ Nuclear Envelope Protein Extraction Kit is the first commercial kit designed to rapidly isolate nuclear envelope and its associated proteins in native form without using density-gradient and ultracentrifugation. Due to the use of protein extraction filter cartridges, the nuclear envelope protein isolation is simple and user-friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional methods that require large amounts of starting cells/tissues, this kit starts with only 10-20 million cells, and the buffers are detergent and EDTA free.
Invent Biotechnologies Inc Minute™ Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) - Spin Column-Based (20 Preps)
Nucleoplasm, also referred to as karyoplasm/nucleus sap, consists of chromosomes, nucleolus and nuclear matrix. Nucleoplasm plays a central role in genetic information flow and regulation of gene expression. Nuclear protein isolation has traditionally been accomplished by extraction protocol using detergent-containing buffers, which is effective in extracting total nuclear proteins but unable to separate membrane-bound proteins and water-soluble matrix proteins. The detergent-free nuclear matrix protein extraction kit is designed to separate cellular protein into three fractions: cytosolic, nuclear matrix and water-insoluble fractions (mainly envelope and nucleic acid-associated proteins) using a patented spin-column-based technology. The buffers used are detergent and EDTA-free. The proteins extracted are in their native state and can be used in many downstream applications such as gel retardation, transcription factor analysis, apoptosis and protein trafficking studies.
Invent Biotechnologies Inc Minute™ High-Efficiency Exosome Precipitation Reagent - Non-PEG (20 ml)
Exosomes are vesicles secreted by cells. They are present in a variety of body fluids such as serum, ascites, spinal cord fluid, urine, and saliva. Cultured cells also secrete significant number of exosomes. The size distribution of exosome is ranging from 30-120 nm. The biological function of exosomes is believed to serve as intercellular messengers. Filtration and ultracentrifugation are classical ways of isolating and enriching exosomes. This is a tedious process and requires special equipment. Another common way for enrichment of exosome is through precipitation. Currently major commercial kits are PEG-based. EI-027 is designed to precipitate total exosomes from biofluids using a high efficacy, non-PEG based reagent for exosome precipitation. This kit is suitable for routine biofluid samples using the same reagent and similar protocol. More information: https://inventbiotech.com/products/minute%E2%84%A2-hi-efficiency-exosome-precipitation-reagent?_pos=1&_sid=c6bc0e161&_ss=r
Invent Biotechnologies Inc Minute™ Adipose Tissue Fractionation Kit - Spin Column-Based (20 Preps)
On average, total cellular proteins account for less than 2% of adipose tissue. Adipose tissue, especially white adipose tissue, has been recognized as an important endocrine and inflammation organ in addition to its energy storage function. Fractionation and analysis of proteins from adipose tissue are critical for understanding many physiological/pathological conditions. However, fractionation of adipose tissue is challenging due to its high lipid and low protein contents. The water-oil emulsion present in biological samples is notoriously difficult to separate, but we have developed a cutting-edge technology to deal with this issue: a porous filter with unique surface property and predefined pore size coupling that has a specially formulated detergent-free fractionation buffer.
Hampton Research CRYSTAL SCREEN NO49 185ML
HR2-910-49/Crystal Screen #49, 185 ml - 1.0 M Lithium sulfate monohydrate, 2% w/v Polyethylene glycol 8,000. Please note items from this supplier are non-returnable.
Hampton Research CRYSTAL SCREEN NO50 185ML
HR2-910-50/Crystal Screen #50, 185 ml - 0.5 M Lithium sulfate monohydrate, 15% w/v Polyethylene glycol 8,000. Please note items from this supplier are non-returnable.
Hampton Research CRYSTAL SCREEN 2 NO06 185ML
HR2-912-06/Crystal Screen 2 #06 (E06), 185 ml - 1.0 M Imidazole pH 7.0. Please note items from this supplier are non-returnable.
Hampton Research CRYSTAL SCREEN 2 NO02 185ML
HR2-912-02/Crystal Screen 2 #02 (E02), 185 ml - 0.5 M Sodium chloride, 0.01 M Magnesium chloride hexahydrate, 0.01 M Hexadecyltrimethylammonium bromide. Please note items from this supplier are non-returnable.
Hampton Research CRYSTAL SCREEN NO45 185ML
HR2-910-45/Crystal Screen #45 (D9), 185 ml - 0.2 M Zinc acetate dihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 18% w/v Polyethylene glycol 8,000. Please note items from this supplier are non-returnable.
Hampton Research CRYSTAL SCREEN 2 NO15 185ML
HR2-912-15/Crystal Screen 2 #15 (F03), 185 ml - 0.5 M Ammonium sulfate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Lithium sulfate monohydrate. Please note items from this supplier are non-returnable.
Hampton Research SALTRX 2 NO39 185ML
HR2-909-39/SaltRx 2 #39 (H03), 185 ml - 0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5. Please note items from this supplier are non-returnable.
Hampton Research SALTRX 2 NO21 185ML
HR2-909-21/SaltRx 2 #21 (F09), 185 ml - 0.8 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5. Please note items from this supplier are non-returnable.
Hampton Research HR2-909-05/SaltRx 2 #05 (E05), 185 ml - 1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0
HR2-909-05/SaltRx 2 #05 (E05), 185 ml - 1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0. Please note items from this supplier are non-returnable.